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Bioinformatics With Galaxy - Metagenomics Analysis Using Galaxy

The basics of 16S bacterial metagenomics analysis

Date: 11 August 2020
Time: 9:00 AM - 5:00 PM
Venue: Online
Contact: For more information, please visit the QCIF website.
Booking: Please register via Eventbrite.
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This workshop will cover the concepts of 16S bacterial metagenomics analysis. You will learn about pre-processing and quality -filtering of your data, assembly of reads into Operational Taxonomic Units (OTUs), and visualisation and export of data for further analysis.

The workshop will be based in Galaxy, a platform that provides a simple and user-friendly interface to bioinformatics tools.

Recommended Participants

Biologists and other life scientists planning to work with next-generation sequencing data from 16S metagenomics samples. No previous bioinformatics experience is required.

Learning Objectives

  • Perform QC evaluation and filtering on next-generation metagenomic sequence data
  • Use the Galaxy interface to classify sample sequences into OTUs
  • Visualise OTUs between samples in an interactive Krona plot
  • Exporting of OTUs and Krona plots for further analysis

Syllabus

  • What is Metagenomic analysis and what types of results can be generated
  • Quality control and filtering of next-generation sequencing data
  • Mapping of sequence data to known microbial databases
  • Clustering and classification of each sequence into OTUs
  • Visualisation of OTUs on a Krona plot